Global Changes Of Transcriptomes Between 3-month-old And 1-year-old Human Right Atria
Shanghai Children's Medical Center, Shanghai China, China.
Background: Young hearts, which contain more cell cycle active cardiomyocytes (CMs) than old hearts, were easier to recover than old hearts after injury. However, the molecular mechanisms that govern the transition of postnatal CM cell cycle changes in human are poorly understood. Methods: Twenty right atrial specimens obtained from children with ventricular septal defect (VSD) during routine congenital cardiac surgery were grouped according to their age (< 3 months or > 1 year) and subjected to RNA-sequencing(RNA-seq) The results showed there were four times as many cell cycle active CMs in the < 3-month group than the > 1-year group. RNA-seq data showed that there were 344 differentially expressed genes between these two groups, of which 119 were upregulated and 225 were downregulated. Gene ontology (GO) analysis showed that the most enriched GO terms included regulation of growth, fatty or lipid acid oxidation and extracellular matrix (ECM). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed the most enriched GO terms were protein digestion and absorption, ECM-receptor interaction and tyrosine metabolism. Results: When human induced pluripotent stem cells (iPSC)-cardiomyocytes (CMs) were treated with atrium ECM extraction, only the 3-month-old ECM extraction increased cell cycle activities of CMs. Conclusions: The change of ECM and metabolism significantly contributed to the postnatal CM's cell cycle activity arrest in human. Human transcriptome changes between 3-month-old and 1-year-old human right atria are different from the changes between P3 and P10 mouse hearts regarding the most enriched GO terms.
Back to 2020 Display ePosters