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Immunohistochemistry comparing endoscopic vein extraction vs. open vein harvesting on saphenous vein endothelium
Mohammad H. Nezafati1, Pouya Nezafati2.
1Javad ol Aeme hospital, mashhad, Iran, Islamic Republic of, 2Student Research Committee, Mashad University of Medical Sciences, mashhad, Iran, Islamic Republic of.
Objective: The present study attempts to compare the immunohistochemistry of vWf, e-cadherin, Caveolin and eNOS in VasoView endoscopic saphenous vein versus traditional open vein harvesting techniques for Coronary Artery Bypass Graft (CABG) Surgery performed in Javad ol Aemeh Hospital of Mashhad, Iran in 2013,.
Methods and Materials: forty-seven patients were scheduled for CABG (30 EVH and 17 OVH) among whom patients with relatively same sex and similar age were selected. Three separate two cm vein samples were harvested from each patient’s saphenous vein. Each portion was collected from distal, middle and proximal zones of the saphenous vein. The tissues were deparaffinized, and antigen retrieval was done using EZ-retriever followed by an immunohistochemistry evaluation with vWf, e-cadherin, Caveolin and eNOS. Data analyses, including parametric and nonparametric tests were undertaken using the SPSS 16 software.
Results:The mean age of the EVH and OVH groups were 63.86±10.16 and 63.91±9.29 years respectively with no significant difference between them (p=0.989). In addition, there was no great difference between the EVH and OVH in lipid profile, DM, HTN, smoking history, CVA, and Valvular dysfunction (p>0.05). Qualitative report of vWf, e-cadherin, Caveolin and eNOS reveals no significant difference between the EVH and OVH (p>0.05)(Figure 1).
Conclusion: This study indicates that VasoView endoscopic saphenous EVH technique causes no endothelial damage in comparison with OVH. This study could be a molecular confirmation for the innocuous of EVH technique.
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